مسح فيروس Y البطاطا وسلالاته في حقول إنتاج البطاطا في محافظة اللاذقية, سورية
Abstract
هدفت هذه الدراسة إلى التحري عن فيروس واي البطاطا (PVY) وسلالاته في عينات نباتية جمعت من حقول البطاطا في اللاذقية-سورية من مواقع صنوبر جبلة, نهر العرب, الشراشير, البصة, بوقا. استخدم في الدراسة اختبار البصمة النسيجية المناعية المباشر (TBIA) والأجسام المضادة وحيدة الكلون لفيروس PVY وسلالاته PVYN, PVYC, PVYC/O. أشارت نتائج الاختبار إلى تباين نسب انتشار الفيروس PVY وسلالاته باختلاف المواقع المدروسة. بلغت نسبة العينات المصابة بالفيروس PVY 65.3% من العينات الكلية, وبلغت نسبة العينات التي تفاعلت مع الأجسام المضادة للسلالات 77.5%, في حين 24.5% من العينات لم تتفاعل مع أي من الأجسام المضادة للسلالات. كما تباينت نسبة السلالات الفيروسية في العينات المختبرة بحالة فردية (PVYN بنسبة 48.6%, PVYO بنسبة 35.1%) أو كإصابات مختلطة بسلالتين ولم يتم الكشف عن وجود إصابة مفردة بالسلالة PVYC.
The aim of this study is to survey Potato virus Y (PVY) strains in potato production fields in Lattakia province, Syria; Sanaober Jablah, Naher Alarab, Sharasher, Bassah, and Bouka sites. Direct tissue blot immunobinding assay (TBIA) and monoclonal antibodies against PVY and PVY strains (PVYN, PVYC, PVYC/O) were used in this study. Results revealed differences in distribution rates of PVY and its strains among surveyed sites. About 65.33% of total tested samples were PVY infected, and 77.5% of these reacted positively with monoclonal antibodies against PVY strains, whereas 24.5 % reacted negatively. Single infection with one PVY strain has been detected in deferent rates, 48.6% for PVYN and 35.1% for PVYO. Mixed infection with PVY strains was also detected. Single infection with PVYC strain has not been found.
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