In vitro response of potato spunta (Solanum tuberosum L.) cultivar for micropropagation under several concentrations of sucrose

Abstract

The experiment was conducted in the Plant Tissue Culture Laboratory of the General Organization for Seed Multiplication in Tartous during the 2023 growing season. The study aimed to investigate the effect of adding different concentrations of sucrose to the micropropagation medium of potato ( Solanum tuberosum L.) meristem tips.

 Four sucrose treatments were used. Green shoots of the cultivar Spunta were taken as the source of meristem tips, sterilized, and cultured on MS (Murashige and Skoog) medium supplemented with the plant growth regulator 6-benzylaminopurine (BAP) at a uniform concentration of 5 mg/L for all treatments except the control. The treatments were as follows: T1 (control): MS medium containing 30 g/L sucrose; T2, T3, and T4: MS medium supplemented with 40, 60, and 80 g/L sucrose, respectively.

The results showed significant differences among the treatments for several vegetative parameters. Treatment T2 exhibited a significant superiority over all other treatments in terms of multiplication rate, shoot length, fresh weight of the vegetative part, and number of shoots per plantlet. In contrast, treatment T4 recorded the lowest values for all the aforementioned traits.