A Comparison between two type of cyanobacteria culture media used for growing of Anabaena variabilis in laboratory.

Abstract

The cyanobacteria Anabaena variabilis were cultured in a two different types of  culture  media, namely the (BG-11) Blue-Green medium11 widely used worldwide, and another modified medium is indicated by the symbol the Modified Cyanobacteria Culture Medium (MCC) in which micro elements were dispensed with and replaced with fertile agricultural soil filtrate, which was sterilized and analyzed. The number of cells (biomass) and the amount of chlorophyll (chl.a, chl.b) in the bacterial cell were compared in the two types of culture media used under the same other growing conditions.

     The number of cells of A. variabilis  in the (MCC) modified medium in the day 12 reached about (10⁷ cells/ml) and was higher than that of the cells in the (BG-11) medium, which reached (10⁶ cells/ml). The amount of chlorophyll (chl.a) in the (MCC) modified medium in the day 12 reached about (0.97mg/ml) and was higher (0.72 mg/ml) than its counterpart  in the (BG-11) medium, and the same was true for chlorophyll (chl.b),  as the amount reached  (0.45mg/ml) in the (MCC) modified medium,  compared to (0.39 mg/ml) for (BG-11) medium.