Antibacterial and Antioxidant Activity of Essential Oil of Wild Ruta Chalepensis
Abstract
Leaves of wild Ruta chalpensis were collected from Salib al-Turkman in Lattakia. Essential oil of dried leaves was isolated using Clevenger; Methanolic extract was prepared using Soxhlet extractor. The antibacterial activity of extracted essential oil was tested against Gram-positive bacteria such as Staphylococcus aureus and Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa , and Klebsiella. Essential oil showed a very high sensitivity against Klebsiella, a high sensitivity against S. aureus and P. aeruginosa, and a good sensitive against E.coli.
Antioxidant activity of methanolic extract and essential oil of Ruta chalepensis was characterized using 1, 1,-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging assay. The IC50 was (0.091 mg/ml) for methanolic extract and (0.071 mg/ml) for essential oil, this means that the methanolic extract has more antioxidant than essential oil.
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